Trichomonas tenax

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Trichomonas tenax, or oral trichomonas, is a species of trichomonas commonly found in the oral cavity of humans, dogs and cats. Routine hygiene is generally not sufficient to eliminate the parasite, hence its Latin name, meaning "tenacious". The parasite is frequently encountered in periodontal infections, affecting more than 50% of the population in some areas, but it is usually considered insignificant. T. tenax is generally not found on the gums of healthy patients.[1][2] It is known to play a pathonogenic role in necrotizing ulcerative gingivitis and necrotizing ulcerative periodontitis, worsening preexisting periodontal disease.[3] This parasite is also implicated in some chronic lung diseases; in such cases, removal of the parasite is sufficient to allow recovery (Mussaev 1976).

Clinical[edit]

History[edit]

During the early 1900s, prisoners at the San Quentin prison in California were subject to advanced periodontal disease at a rate of almost 90%, owing at least in part to a high rate of infection by T. tenax.[4] In the Journal of the American Dental Association, it was speculated that this high rate of transmission was attributable to the crowding and poor diets faced by inmates. Age is also an important factor (Kofoid and al.1929) and intense inflammation is characteristic. This was one of the very first clinical testings of T. tenax.

Transmission[edit]

T. tenax is a commensal of the human oral cavity, found particularly in the patients with poor oral hygiene and advanced periodontal disease. Transmission is through saliva, droplet spray, and kissing or use of contaminated dishes or drinking water. (11,15)[5]

Symptoms[edit]

T. tenax alone is not known to cause any symptoms. There are merely implications that this parasite may worsen preexisting periodontal disease and in rare cases has been reported to cause bronchopulmonary infections, mainly in patients with underlying cancers or other lung diseases.(4,6,9,11,13,15-17)[6] The organism is believed to enter the respiratory tract by aspiration from the oropharynx.

Life Cycle[edit]

T. tenax trophozoites survive in the body as mouth scavengers that feed primarily on local microorganisms located between the teeth, tonsillar crypts, pyoirheal pockets, and the gingival margin around the gums. T. tenax trophozoites multiply by longitudinal binary fission. These trophozoites are unable to survive the digestive process.

Diagnosis[edit]

The specimen of choice for diagnosing Trichomonas tenax trophozoite is mouth scrapings. Microscopic examination of tonsillar crypts and pyorrheal pockets of patients suffering from T. tenax infections often yields the typical trophozoites. Tartar between the teeth and the gingival margin of the gums are the primary areas of the mouth that may also potentially harbor this organism. T. tenax may also be cultured onto appropriate media.

Treatment[edit]

Regardless of patient’s demographic characteristics, it seems that oral hygiene instructions in combination with scaling and root planning can help with controlling excessive colonization of parasites, particularly E. gingivalis and T. tenax and their probable opportunistic infestation.[7] T. tenax can easily be detected through the use of phase contrast microscopy. Biofilm harvested from infested areas of the periodontal pockets can be mounted onto a slide; T. tenax, if present, will be clearly visible. The preparation must use the patient's saliva as the medium, as the use of running water or saline could cause warping.

Morphology[edit]

Of the three parasites in the genus Trichomonas, T. tenax is the smallest, measuring only 5-14 µm and 6-9 µm wide; specimens can be identified by their long axostyles and tails, 4 anterior flagella, and by the recurrent flagellum that raises an undulating membrane which is two thirds the length of the body. This undulating membrane may appear like small legs. It may occasionally appear larger, allowing it to be confused with Trichomonas vaginalis due to similar morphology. In such cases, the presence of an oral or vaginal parasite should be confirmed, due to the ease with which the parasite can be transmitted through direct contact of mucous membranes.

Protein Function[edit]

In infected hosts, the parasite can typically be found among dental calculus, as well as within the tonsillar crypts, which will often become purulent during the course of infection. T. tenax may also be involved in the degradation of periodontal tissue through the secretion of substances such as alkaline phosphatases and the fibronectin cathepsine.[8] T. tenax is classified as a parasite due to the manner in which it causes damage to host tissues; its behavior when in contact with target cells is similar to the closely related and likewise parasitic T. vaginalis.[9] It has no cysts and is transmitted directly from its vegetative form.

Further Research[edit]

Cultural methods were studied under different conditions in order to acquire a large number of T. tenax for the need of clinical diagnosis and theoretical study. The positive rate of T. tenax by culture was higher than that by direct examination. The reproductive effect of T. tenax was better in LES medium. pH value between 5.80 and 7.00 with cultivation temperature of 35 °C than other media with pH 5.40 and cultivation temperature of 37 °C.[10] Normal, healthy human saliva is slightly alkaline at pH 7.40 just like blood. Whitening products have a mean pH of 8.22 with a range (5.09-11.13). Whitening toothpastes have a mean pH of 6.83 with a range (4.22-8.35). Mouthwashes also vary greatly in pH from pH 4.40 to 6.80. Mouth rinsing after an acidic challenge increased salivary pH. The tested mouthwashes raised pH higher than water. Mouthwashes with a neutralizing effect can potentially reduce tooth erosion from acid exposure.[11] Essentially the human mouth is an optimum habitat for this organism and any pH level alteration needed to stunt the reproductive rate significantly would require a duration of time that would cause greater damage to the tooth enamel than to T. tenax. Regular oral hygiene and dental visits to remove dental plaque is currently the best viable solution to deal with this protozoan.

References[edit]

  1. ^ T. Lyons et al. "Oral amoebiasis: a new approach for the General Practitioner in the diagnosis and the treatment of periodontal disease", Oral Health 1980, 70:39-41, 108, 110.
  2. ^ T. Lyons Introduction to protozoa and fungi in periodontal infections. Trevor Lyons Publications, Ontario, Canada 1989. ISBN 0-9693950-0-0
  3. ^ Bonner M. To Kiss or Not to Kiss. A cure for gum disease. Amyris Editions, 2013 EAN : 978-28755-2016-6
  4. ^ Kofoid CA, Hinshaw HC, HG Johnsotne. "Animal parasites of the mouth and their relation to dental disease," Journal of the American Dental Association 1929 1434-1455
  5. ^ Mallat, Hassan; Podglajen, Isabelle; Lavarde, Véronique; Mainardi, Jean-Luc; Frappier, Jérôme; Cornet, Muriel (2004-08-01). "Molecular Characterization of Trichomonas tenax Causing Pulmonary Infection". Journal of Clinical Microbiology. 42 (8): 3886–3887. doi:10.1128/JCM.42.8.3886-3887.2004. ISSN 0095-1137. PMC 497589. PMID 15297557.
  6. ^ Mallat, Hassan; Podglajen, Isabelle; Lavarde, Véronique; Mainardi, Jean-Luc; Frappier, Jérôme; Cornet, Muriel (2004-08-01). "Molecular Characterization of Trichomonas tenax Causing Pulmonary Infection". Journal of Clinical Microbiology. 42 (8): 3886–3887. doi:10.1128/JCM.42.8.3886-3887.2004. ISSN 0095-1137. PMC 497589. PMID 15297557.
  7. ^ Rashidi Maybodi, Fahimeh; Haerian Ardakani, Ahmad; Fattahi Bafghi, Ali; Haerian Ardakani, Alireza; Zafarbakhsh, Akram (September 2016). "The Effect of Nonsurgical Periodontal Therapy on Trichomonas Tenax and Entamoeba Gingivalis in Patients with Chronic Periodontitis". Journal of Dentistry. 17 (3): 171–176. ISSN 2345-6485. PMC 5006825. PMID 27602391.
  8. ^ Trichomoniasis Trichomonas tenax pleural. Porcheret, Maisonneuve, Esteve, Jagot, Le Pennec. 2002
  9. ^ Ribeiro LC, Santos C, Benchimol M. Is Trichomonas tenax a Parasite or a Commensal? Protist 2015
  10. ^ Chen, Jinfu; Liu, Guangying; Zeng, Guoqi; Wen, Wangrong (1997). "Trichomonas tenax culture in vitro". Journal of Fujian Medical College. 31 (2). ISSN 1000-2235.
  11. ^ Dehghan, Mojdeh; Tantbirojn, Daranee; Kymer-Davis, Emily; Stewart, Colette W.; Zhang, Yanhui H.; Versluis, Antheunis; Garcia-Godoy, Franklin (May 2017). "Neutralizing salivary pH by mouthwashes after an acidic challenge". Journal of Investigative and Clinical Dentistry. 8 (2). doi:10.1111/jicd.12198. ISSN 2041-1626. PMID 26616243.

Further reading[edit]

Dimasuay, Kris Genelyn B.; Rivera, Windell L.; Fontanilla, Dr. Ian Kendrich C. (April 2014). "First report of Trichomonas tenax infections in the Philippines". Parasitology International. 63 (2): 400–402. doi:10.1016/j.parint.2013.12.015. PMID 24406842. Retrieved 11 February 2015.